Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods

doi:10.7523/j.issn.2095-6134.2007.1.017

›› 2007, Vol. 24 ›› Issue (1): 114-118.DOI: 10.7523/j.issn.2095-6134.2007.1.017

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Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods

LU Yang, PENG Bi-Xian

Technical Institute of Physics and Chemistry, Chinese Academy of Science

Graduate University of the Chinese Academy of Science

Received:1900-01-01 Revised:1900-01-01 Online:2007-01-15

Abstract

Abstract:

Isoelectric point (IEP) is one of the most important physicochemical properties of protein. There are many different methods for the determination of IEP. In this paper, a comparative study between the simplest turbidimetry/spectrometry method and the most advanced capillary IEP focusing (CaIPF) method was made. Henan cattle hide gelatin’s IEP was determined with the two methods and the advantages of the CaIPF method over the other methods were demonstrated and discussed.

Key words: Isoelectric point, gelatin, turbidimetry/spectrometry, capillary IEP focusing

CLC Number:

LU Yang, PENG Bi-Xian.

Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods

[J]. , 2007, 24(1): 114-118.

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Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods

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